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I just got this done today ten injections so 100ml as it was first time and the pain sweet mother of Christ the pain ! I have an extremely high pain tolerance and found this rather painful and teary I have to get this done every 6 months and upped to 200ml hioebit works and was worth the pain during and after I feel sick to my stomach and in agony so here's hoping it works it takes two weeks to start becoming affective